RESUMO
Five types of honey (multifloral, sunflower, linden, rapeseed, and acacia), from Southern Romania, were classified using chemometrics methods coupled with IR spectroscopy. The botanical origin's effect on the physicochemical characteristics of honey was studied to highlight the most valuable plant source of honey. Except for antioxidant activity, the moisture, ash, electrical conductivity (EC), pH, free acidity (FA), total sugar content (TSC), hydroxymethylfurfural (HMF), total phenolic (TPC), tannin (TTC), and flavonoid content (TFC) were significantly influenced by the botanical origin of the honey. The results showed that sunflower honey had the highest moisture (15.53%), free acidity (16.67 mEq kg-1), electrical conductivity (483.92 µS cm-1), phenolics (167.59 mg GAE 100 g-1), and flavonoids (19.00 mg CE 100 g-1), whereas multifloral honey presented the highest total sugar content (69.64 g Glu 100 g-1). The highest HMF content was found in linden honey (33.94 mg kg-1). The HMF contents of all tested honey were within the standard recommended limit, and they confirmed that the tested honey was free of any heat treatment. All five types of tested honey presented a safe moisture content for storage and consumption (12.21-18.74%). The honey's free acidity was in the range of 4.00 to 25.00 mEq kg-1; this indicated the freshness of the samples and the absence of any fermentation processes in the tested honey. Honey with a total sugar content over 60% (except for linden honey, with 58.05 g glucose 100 g-1) showed the characteristic of nectar-derived honey. The elevated antioxidant activity of honey was correlated with its high moisture, flavonoids, and HMF, whereas the tannins and HMF were positively correlated with ash and electrical conductivity. The higher content of phenolics, flavonoids, and tannins was correlated with higher free acidity. The chemometric method, coupled with ATR-FTIR spectra, revealed a clear separation between linden honey from acacia, multifloral, and sunflower honey.
RESUMO
The popularity of Vaccinium corymbosum blueberry cv. has increased over time because its fruits are highly valued for their taste, aroma, and multiple ways of use. A field trial with two genotypes and their hybrids was organized during 2021-2022 at the Research Institute for Fruit Growing Pitesti-Maracineni, Romania. This paper proposes a comparative analysis of the quality of berries in 17 hybrids of the 'Simultan' and 'Duke' cultivars, selected by the size and the soluble solid content, in agreement with the objectives of the blueberry breeding program. The genotype influence on berry weight, total soluble solids, pH, vitamin C, total polyphenols, total flavonoids, total anthocyanins, lycopene, ß-carotene, and antioxidant activity was determined considering the climatic factors. The results showed that fruit weight varied between 1.22 and 2.47 g, total soluble solids reached a maximum of 19.22 °Brix, and the pH oscillated between 3.14 and 3.89. Vitamin C content varied from 9.52 to 18.69 mg in 100 g fresh weight, with an average of 14.35 mg/100 g. Total polyphenol, flavonoid, and anthocyanin contents averaged 709.92 mg gallic acid equivalent in 100 g fresh weight, 165.48 mg catechin equivalent in 100 g fresh weight, and 81.88 mg cyanidin-3-O-glucoside equivalent in 100 g fresh weight, respectively. Results show that the strategy of growers to produce blueberries with a large diameter, visually attractive for traders and consumers, is not sufficient for repeat sales. Our study proves that large fruits do not have the highest content of bioactive compounds. Smaller berries had higher polyphenol, lycopene, and ß-carotene contents. It is recommended that the selection of the hybrid in the breeding program also takes into account the content of bioactive compounds.
RESUMO
The emission properties of norfloxacin, a quinolone antibiotic, in presence of salmon sperm DNA were studied at room temperature and in conditions of acid, alkaline and neutral pH. It was found that norfloxacin molecules are inserted between the DNA base pairs, as evidenced by the emission spectra features and the significant increases in relative viscosity of DNA by the addition of norfloxacin. The fluorescence quenching process was characterized by Stern-Volmer plots which display a positive deviation from the linearity. The analysis was performed in terms of the Stern-Volmer modified equations including both dynamic and static quenching. The use of the finite sink approximation model showed that the process of quenching of the norfloxacin fluorescence with DNA was diffusion limited, irrespective to the pH of the work solution. At the same time, relying on the formation of the ground state complex model and the sphere of action static quenching model, we concluded that the quenching reaction from the norfloxacin - DNA system is due to the combined effect of both dynamic and static quenching processes.
